{"id":"f53391d0-3e5d-4fd3-9084-9c604a4e9317","title":"青藏高原与西北干旱区33个湖泊表层沉积植物DNA数据","title_en":"Data of plant DNA in surface sediments of 33 lakes in Qinghai-Tibetan Plateau and arid northwestern China","serial":"","user_limit":"为尊重知识产权、保障数据作者的权益、扩展数据中心的服务、评估数据的应用潜力，请数据使用者在使用数据所产生的研究成果中（包括公开发表的论文、论著、数据产品和未公开发表的研究报告、数据产品等成果），明确注明数据来源和数据作者。对于转载（二次或多次发布）的数据，作者还须注明原始数据来源。","instructions":"数据为fastq文件，建议使用OBITOOLS等生物信息学工具进行读取并分析，具体可参考：https://datadryad.org/stash/dataset/doi:10.5061/dryad.k6djh9w4r。","description":"数据包括青藏高原与西北干旱区33个湖泊表层沉积物中植物DNA的原始测序文件。我们使用德国Qiagen公司的PowerMax土壤试剂盒提取DNA，并采用通用植物引物g-h (Taberlet et al., 2007) 对样品中叶绿体trnL (UAA) 内含子区的P6环进行PCR扩增，PCR产物随后送至瑞士Fasteris公司进行第二代高通量双端测序，测序仪器为Illumina NextSeq 550。数据质量分数Q30为81.97。","doi":"https://doi.org/10.5061/dryad.k6djh9w4r","projection":"","language":"zh","share_type":"online","east":116.5115,"west":81.2447,"south":29.2074,"north":47.1048,"scale":null,"crs":null,"filesize":3173.0,"share_policy":"a","ts_created":"2021-05-31T09:56:42.359159+08:00","doi_type":"国际公开共享数据","ts_updated":"2021-12-16T16:03:15.027961+08:00","time_description":"现代","datas_group":3,"time_description_en":"modern","data_format":null,"project":[11],"specials":[],"cite":null,"review_status":5,"review_invite":[{"user_name":"杨晓燕","username":"杨晓燕","email":"xyang@itpcas.ac.cn","is_cancel_expert":false}],"review_editor":{"id":42421,"last_login":"2022-04-08T15:16:51.670544+08:00","username":"任慧霞","email":"510727846@qq.com","is_active":true,"is_staff":true,"user_name":"任慧霞"},"data_sources":null,"temporal_resolution":"Yearly","spatial_resolution":"-","license":"Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","fund":[],"subtopics":{"metadata":"f53391d0-3e5d-4fd3-9084-9c604a4e9317","three_pole":null,"third_pole":null},"username":"陈嵘","review_time":null,"protected_duration":null,"protected_describe":null,"is_important":false,"hit_count":54}