{"id":"97c982e9-a94b-4050-bdb9-cbc820a602aa","title":"青藏高原湖水细菌多样性调查（V1.0）（2015）","title_en":"Bacteria distribution in Tibetan lakes (version 1.0) (2015)","serial":"地球大数据科学工程专项时空三极环境项目","user_limit":"为尊重知识产权、保障数据作者的权益、扩展数据中心的服务、评估数据的应用潜力，请数据使用者在使用数据所产生的研究成果中（包括公开发表的论文、论著、数据产品和未公开发表的研究报告、数据产品等成果），明确注明数据来源和数据作者。对于转载（二次或多次发布）的数据，作者还须注明原始数据来源。","instructions":"","description":"青藏高原湖水微生物多样性数据。样品采集时间为2015年7月1日至7月15日，包含28个湖泊(巴木措,白马湖,班戈盐湖,班公湖,崩错,别若则错,错萼措,错愕（平措北）,达瓦措,当穹错,当惹雍措,洞措,鄂雅错琼,公珠措,果根错,甲热布错,玛旁雍错,纳木错,聂尔错（盐湖）,诺尔玛措,朋彦错,蓬错,枪勇,色林错,吴如错,物玛错,扎日南木措,扎西措,)，138个样品。盐度梯度为0.07-118 ppm。\nDNA提取方法：湖水过滤到0.45膜上，然后通过MO BIO PowerSoil DNA试剂盒提取DNA。16S rRNA基因片段扩增引物为515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 909r (5'-GGACTACHVGGGTWTCTAAT-3')。测序方式为Illumina MiSeq PE250，原始数据通过Mothur软件分析，包括quality filtering, chimera removal, 序列分类依据Silva109数据库，古菌、真核和未知来源序列已被移除。OTU以97%相似度分类，然后只在数据库中出现一次的序列被移除。最后每个样品被重取样到7，230序列/样品。\nGPS坐标,进化信息，环境因子见数据内。","doi":"10.11888/Lake.tpe.0000009.file","projection":"","language":"zh","share_type":"online","east":90.227033,"west":88.688369,"south":32.930061,"north":28.889067,"scale":null,"crs":"","filesize":3.0,"share_policy":"a","ts_created":"2018-02-13T14:08:02+08:00","doi_type":"","ts_updated":"2021-04-19T13:16:37.539000+08:00","time_description":"","datas_group":3,"time_description_en":"","data_format":"txt","project":[10,11],"specials":["1832ecb3-70d0-4b5e-bdb5-2e9ff4ad1efe","f2a78cde-1c98-4241-94dc-7532eba32b80"],"cite":"王磊, 计慕侃. 青藏高原2015年7月湖水细菌多样性调查（V1.0）. 时空三极环境大数据共享与集成平台. 2018","review_status":5,"review_invite":[],"review_editor":{"id":21057,"last_login":"2022-04-02T11:43:37.753860+08:00","username":"gecm","email":"gechm@lzb.ac.cn","is_active":true,"is_staff":true,"user_name":"盖春梅"},"data_sources":"","temporal_resolution":"Yearly","spatial_resolution":"","license":"Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","fund":[5,21],"subtopics":{"metadata":"97c982e9-a94b-4050-bdb9-cbc820a602aa","three_pole":"XDA19070304","third_pole":null},"username":"计慕侃","review_time":null,"protected_duration":6,"protected_describe":null,"is_important":false,"hit_count":2517}