{"id":"7a235ce0-6b9b-4741-aba8-1cf087143bbb","title":"青藏高原冰川、径流、冰川前缘土壤微生物样品采集照片（2019）","title_en":"Photos of soil microbial samples collected from glaciers, runoff and glacier front in Qinghai Tibet Plateau (2019)","serial":"","user_limit":"为尊重知识产权、保障数据作者的权益、扩展数据中心的服务、评估数据的应用潜力，请数据使用者在使用数据所产生的研究成果中（包括公开发表的论文、论著、数据产品和未公开发表的研究报告、数据产品等成果），明确注明数据来源和数据作者。对于转载（二次或多次发布）的数据，作者还须注明原始数据来源。","instructions":"yyyymm-site-type-order.jpg  \n采样年份月份-采样点-样品类型-序号.jpg","description":"记录冰川、径流、土壤及空气微生物样品的原始采集过程。1）冰雪微生物样品采集：采集过程中佩戴洁净手套，将冰雪采集至洁净自封袋中。2）冰尘微生物样品采集：通过软管插入冰尘穴底部，使用注射器将底泥、融水吸入到采样瓶中，低温保存带回实验室，底泥和冰尘穴上部融水均用来提取环境DNA。3）径流包括冰上径流和冰川前缘径流，直接采集径流融水至采样瓶或者采水袋中。4）冰川前缘土壤的采集：使用铲子采集土壤样品，通过2mm土筛后将土壤装至洁净的whirl-pak采样袋中，然后低温保存，用于后续土壤DNA的提取。5）空气膜样品采集：将设计好的采样装置置于采样点，装置下部为电池（可持续工作48h)，上部包含两张滤膜收集空气微生物，用于DNA的提取。6）冰川径流、融水中理化性质的实时监测，使用YSI多参数水质仪，直接放入待测样品中，获取温度、DO、叶绿素浓度等。","doi":"10.11888/Glacio.tpdc.271750","projection":"","language":"zh","share_type":"online","east":96.934,"west":86.851,"south":28.0225,"north":33.11,"scale":null,"crs":null,"filesize":66.38,"share_policy":"a","ts_created":"2021-10-01T15:01:54.565435+08:00","doi_type":"拥有自主知识产权","ts_updated":"2021-11-02T16:19:10.650457+08:00","time_description":"","datas_group":3,"time_description_en":"","data_format":null,"project":[11],"specials":[],"cite":null,"review_status":5,"review_invite":[{"user_name":"侯居峙","username":"houjuzhi","email":"houjz@itpcas.ac.cn","is_cancel_expert":false}],"review_editor":{"id":42421,"last_login":"2022-04-08T15:16:51.670544+08:00","username":"任慧霞","email":"510727846@qq.com","is_active":true,"is_staff":true,"user_name":"任慧霞"},"data_sources":null,"temporal_resolution":"Monthly","spatial_resolution":"10km - 100km","license":"Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","fund":[21],"subtopics":{"metadata":"7a235ce0-6b9b-4741-aba8-1cf087143bbb","three_pole":null,"third_pole":null},"username":"邢婷婷","review_time":null,"protected_duration":null,"protected_describe":null,"is_important":false,"hit_count":428}