{"id":"41ade473-a6d1-462e-9f2e-36932ddc8fb4","title":"现代藏族人群全基因组变异数据（2020）","title_en":"Genomic variation data of modern Tibetans (2020)","serial":"","user_limit":"为尊重知识产权、保障数据作者的权益、扩展数据中心的服务、评估数据的应用潜力，请数据使用者在使用数据所产生的研究成果中（包括公开发表的论文、论著、数据产品和未公开发表的研究报告、数据产品等成果），明确注明数据来源和数据作者。对于转载（二次或多次发布）的数据，作者还须注明原始数据来源。","instructions":"以文本格式储存，文件名称为xxxx.vcf，xxxx表示样本编号。\n数据读取方式：可以直接用文本程序（如Editplus）打开。","description":"我们获得了30个藏族个体的全基因组变异数据开展研究，采用DNA 微阵列（DNA Array）的方法，对30个样本进行了SNP分型检测，获得每个样品约70万位点（包括核基因组、线粒体DNA和Y染色体）分型结果。首先，在提取基因组DNA后，进行DNA扩增、酶促片段化、沉淀和重悬。随后样品过夜孵育过程中和BeadChip杂交，DNA经退火得到位点特异性的50-mer探针，与某种Infinium微珠类型共价偶联。然后采用Infinium XT继续酶基延伸赋予等位基因特异性，然后进行荧光染色。采用iScan系统检测微珠的荧光其强度，Illumina软件自动执行分析和基因型识别。最后得出每个样本的SNP分型结果。基于上述数据，进行相关生物信息分析（主要包括芯片位点质控分析、Y染色体和线粒体DNA的单倍群分型分析）。结合上年度的数据，有助于从核基因组、Y染色体和线粒体DNA的角度，解析藏族人群的遗传结构，通过与高原周边人群数据的比较，可以较为全面地追溯高原人群的迁徙和定居历史。","doi":"10.11888/Ecolo.tpdc.271051","projection":"","language":"zh","share_type":"protected","east":89.70133157226564,"west":87.14151711914064,"south":28.74123370305203,"north":29.470748928891506,"scale":null,"crs":null,"filesize":1208.0,"share_policy":"c","ts_created":"2020-12-22T07:09:58.996694+08:00","doi_type":"拥有自主知识产权","ts_updated":"2021-04-19T11:19:42.772619+08:00","time_description":"","datas_group":0,"time_description_en":"","data_format":null,"project":[11],"specials":["f2a78cde-1c98-4241-94dc-7532eba32b80"],"cite":null,"review_status":5,"review_invite":[{"user_name":"于黎 ","username":"于黎","email":"yuli@ynu.edu.cn","is_cancel_expert":false}],"review_editor":{"id":42421,"last_login":"2022-04-08T15:16:51.670544+08:00","username":"任慧霞","email":"510727846@qq.com","is_active":true,"is_staff":true,"user_name":"任慧霞"},"data_sources":null,"temporal_resolution":"Yearly","spatial_resolution":"1km - 10km","license":"Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","fund":[6],"subtopics":{"metadata":"41ade473-a6d1-462e-9f2e-36932ddc8fb4","three_pole":null,"third_pole":"XDA20040102"},"username":"XDA20040102","review_time":null,"protected_duration":24,"protected_describe":null,"is_important":false,"hit_count":1398}